Toxin detection in patients' sera by mass spectrometry during two outbreaks of type A Botulism in France.
نویسندگان
چکیده
In two outbreaks of food-borne botulism in France, Clostridium botulinum type A was isolated and characterized from incriminated foods. Botulinum neurotoxin type A was detected in the patients' sera by mouse bioassay and in vitro endopeptidase assay with an immunocapture step and identification of the cleavage products by mass spectrometry.
منابع مشابه
گزارش 4 مورد بوتولیسم غذائی در یک خانواده
Botulism is caused by a neurotoxin produced from the anaerobic, Spore-forming bacterium clostridium botulinum. Botulism have four clinical forms:1)Food –borne botulism 2)Wound botulism 3)Infant botulism 4)undetermined botulism, that the most common is food-borne botulism.  ...
متن کاملDetection, differentiation, and identification of botulinum neurotoxin serotypes C, CD, D, and DC by highly specific immunoassays and mass spectrometry.
Botulinum neurotoxin (BoNT) serotypes C and D and their mosaic variants CD and DC cause severe cases of botulism in animal husbandry and wildlife. Epidemiological data on the exact serotype or toxin variant causing outbreaks are rarely available, mainly because of their high sequence identity and the lack of fast and specific screening tools to detect and differentiate the four similar toxins. ...
متن کاملType A botulism outbreak in members of a family following consumption of homemade whey: brief report
Background: Botulism is mostly caused by Clostridium botulinum neurotoxin which has been described as a bilateral symmetric descending flaccid paralysis. Preventing and responding to botulism outbreaks is a public health emergency. In this study, the disease is reported in a family. Methods: In a case series study, during an outbreak, four members of a family with symptoms including paralysis,...
متن کاملBotulism Outbreak in a Family after Ingestion of Locally Produced Cheese
Botulism is one of the most important foodborne diseases and is caused by Clostridium botulinum toxin. The main manifestations are flaccid muscle paralysis and cranial nerve palsies. Botulism is an essential health problem because of its high mortality. The diagnosis of botulism, especially in sporadic cases, is a medical challenge and a high clinical suspicion is necessary for early recognitio...
متن کاملMass Spectrometric Detection of Bacterial Protein Toxins and Their Enzymatic Activity
Mass spectrometry has recently become a powerful technique for bacterial identification. Mass spectrometry approaches generally rely upon introduction of the bacteria into a matrix-assisted laser-desorption time-of-flight (MALDI-TOF) mass spectrometer with mass spectrometric recognition of proteins specific to that organism that form a reliable fingerprint. With some bacteria, such as Bacillus ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 50 12 شماره
صفحات -
تاریخ انتشار 2012